Role of calcium in activity and stability of the Lactococcus lactis cell envelope proteinase.

@article{Exterkate1999RoleOC,
  title={Role of calcium in activity and stability of the Lactococcus lactis cell envelope proteinase.},
  author={Fred A. Exterkate and Arno C. Alting},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 4},
  pages={1390-6}
}
The mature lactococcal cell envelope proteinase (CEP) consists of an N-terminal subtilisin-like proteinase domain and a large C-terminal extension of unknown function whose far end anchors the molecule in the cell envelope. Different types of CEP can be distinguished on the basis of specificity and amino acid sequence. Removal of weakly bound Ca2+ from the native cell-bound CEP of Lactococcus lactis SK11 (type III specificity) is coupled with a significant reversible decrease in specific… CONTINUE READING