Role of calcium-activated neutral protease (calpain) in cell death in cultured neonatal rat cardiomyocytes during metabolic inhibition.

@article{Atsma1995RoleOC,
  title={Role of calcium-activated neutral protease (calpain) in cell death in cultured neonatal rat cardiomyocytes during metabolic inhibition.},
  author={D. E. Atsma and E. M. Lars Bastiaanse and A Jerzewski and L J van der Valk and Arnoud van der Laarse},
  journal={Circulation research},
  year={1995},
  volume={76 6},
  pages={1071-8}
}
Calcium-activated neutral protease (CANP), also known as calpain, has been implicated in the development of cell death in ischemic hearts. CANP is thought to be activated by the calcium overload that develops during ischemia. We studied the involvement of CANP in cell death in cultured neonatal rat cardiomyocytes during metabolic inhibition (5 mmol/L NaCN + 10 mmol/L 2-deoxyglucose). First, we isolated CANP using ion exchange and affinity chromatography. Then the efficacy of the CANP inhibitors… CONTINUE READING