Role of c-kit in mammalian spermatogenesis

  title={Role of c-kit in mammalian spermatogenesis},
  author={Pellegrino Rossi and Claudio Sette and Susanna Dolci and Raffaele Geremia},
  journal={Journal of Endocrinological Investigation},
The tyrosine-kinase receptor c-kit and its ligand, stem cell factor (SCF), are essential for the maintenance of primordial germ cells (PGCs) in both sexes. However, c-kit and a postmeiotic- specific alternative c-kit gene product play important roles also during post-natal stages of spermatogenesis. In the adult testis, the c-kit receptor is re-expressed in differentiating spermatogonia, but not in spermatogonial stem cells, whereas SCF is expressed by Sertoli cells under FSH stimulation. SCF… 
Molecular mechanisms utilized by alternative c‐kit gene products in the control of spermatogonial proliferation and sperm‐mediated egg activation
The most recent findings about the molecular pathways through which c‐kit regulates cell cycle progression in mitotic germ cells, and those through which sperm‐derived tr‐kit triggers parthenogenetic completion of meiosis II and pronuclear formation in microinjected mouse eggs are summarized.
Expression of KIT in the ovary, and the role of somatic precursor cells.
Stage-specific Localization and Expression of c-kit in the Adult Human Testis
Intense protein localization in spermatogonia was detected in stages I–III, whereas low expression was observed in stages IV–VI of the seminiferous epithelium, indicating that the expression of the molecule was stage specific.
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis
It is demonstrated here that PKnox1 is required for adult spermatogenesis in a germ cell-intrinsic manner, andTamoxifen-mediated P Knox1 loss in the adult testes causes testis hypotrophy with germ cell apoptosis and, as a consequence, compromised sperMatogenesis.
Expression of a truncated form of KIT tyrosine kinase in human spermatozoa correlates with sperm DNA integrity.
The TR-KIT is present in the equatorial region of human spermatozoa, which is the first sperm component entering into the oocyte cytoplasm after fusion with the egg.
In vivo and in vitro differentiation of male germ cells in the mouse.
Researchers were able to isolate germ stem cells from embryonic stem cell-derived embryoid bodies, and drive these into gametes either in vivo or in vitro, and the prospects are that this goal is not too far from being accomplished.
Sexually Dimorphic Expression of the Novel Germ Cell Antigen TEX101 During Mouse Gonad Development
Findings indicate that TEX101 is a specific marker for both male and female germ cells during gonadal development, and may play an important physiological role in germ cell development.
Identification of the Leukemia Inhibitory Factor Cell Targets Within the Rat Testis
It is shown that LIF increased [3H]-thymidine incorporation in spermatogonia in microdissected, cultured seminiferous tubules and strongly suggest that Lif has a role in the regulation of the sperMatogonial cell compartment.
New insights into male gametogenesis: what about the spermatogonial stem cell niche?
  • J. Dadoune
  • Biology
    Folia histochemica et cytobiologica
  • 2007
The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and


Expression of the c-kit proto-oncogene in the murine male germ cells.
A role of c-kit not only during testis development in the embryo, but also throughout all stages of male germ cell development after birth is suggested.
Differential expression of c-kit in mouse undifferentiated and differentiating type A spermatogonia.
It is concluded that c-kit can be used as a marker for differentiation of undifferentiated into differentiating type A spermatogonia.
Switching of mouse spermatogonial proliferation from the c-kit receptor-independent type to the receptor-dependent type during differentiation.
The results indicate that c-kit-positive type A spermatogonia in the testes of 5-day-old mice require steel factor (kit ligand) for their proliferation, whereas self-renewal and differentiation of c-Kit-negative primitive type A Sertoli cells from neonatal mice 2 and 5 days old do not.
Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue.
It appears that the c-kit receptor is expressed in the acrosome of developing germ cells, as well as in Leydig cells and early spermatogenic cells, suggesting a role in theAcrosome reaction, aswell as germ cell maturation and differentiation.
A cell- and developmental stage-specific promoter drives the expression of a truncated c-kit protein during mouse spermatid elongation.
Western blot experiments showed that a polypeptide, of the size predicted by the open reading frame of the spermatid-specific c-kit cDNA, accumulates in the latest stages of sperMatogenesis and in epididymal spermatozoa.
Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis.
A complex pattern of expression of the two components in the adult murine testis is identified, suggestive of a role in the meiotic progression of spermatocytes, and of the membrane-associated form of the ligand suggested by the observation that addition of the soluble form of KL was equally inhibitory.
Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF.
To study self-renewal and differentiation of spermatogonial stem cells, we have transplanted undifferentiated testicular germ cells of the GFP transgenic mice into seminiferous tubules of mutant mice
Function of Stem Cell Factor as a Survival Factor of Spermatogonia and Localization of Messenger Ribonucleic Acid in the Rat Seminiferous Epithelium.
The expression of the SCF gene was found to be developmentally regulated, and the expression pattern followed the process of Sertoli cell proliferation and differentiation during postnatal life.
Stem cell factor protects germ cells from apoptosis in vitro.
It is demonstrated that SCF acts as an important survival factor for germ cells in the adult rat testis and FSH pro-survival effect on germ cells is mediated partially through the SCF/c-kit pathway.
Gonadal expression of c-kit encoded at the W locus of the mouse.
It was concluded that the period of expression of c-kit extends from at least as early as type A2 spermatogonia through type B sperMatogonia and into preleptotene sperMATocytes.