Role of adenosine 3′,5′-cyclic monophosphate in adrenoceptor-mediated control of 3H-noradrenaline secretion in guinea-pig ileum myenteric nerve terminals

Abstract

Secretion of 3H-noradrenaline was evoked by electrical field stimulation (1 Hz, 300 shocks) in guinea-pig ileum myenteric plexus. The role of cyclic nucleotides in the presynaptic receptor-mediated control of 3H-noradrenaline secretion was studied. The secretion of 3H-noradrenaline was maximally enhanced to the same extent, viz. 300–400% of control, by two analogues of cyclic AMP (8-Br cyclic AMP and dibutyryl cyclic AMP), by an adenylate cyclase activator (forskolin) and by three structurally different inhibitors of phosphodiesterase (3-isobutyl-1-methylxanthine, SQ 20,006 and Ro 20–1724), but not altered by two analogues of cyclic GMP (8-Br cyclic GMP and dibutyryl cyclic GMP). Added separately an α2-adrenoceptor antagonist (yohimbine) and a β-adrenoceptor agonist (isoprenaline) enhanced the 3H-noradrenaline secretion. Yohimbine, but not isoprenaline, increased additively the ‘maximal enhancement’ of the 3H-noradrenaline secretion caused by 3-isobutyl-1-methylxanthine. These results suggest that neuronal cyclic AMP may be involved in β-but not in α-adrenoceptor-mediated control of 3H-noradrenaline secretion in guinea-pig ileum myenteric nerve terminals.

DOI: 10.1007/BF00499903

7 Figures and Tables

Statistics

050010001500'89'92'95'98'01'04'07'10'13'16
Citations per Year

931 Citations

Semantic Scholar estimates that this publication has 931 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Alberts1985RoleOA, title={Role of adenosine 3′,5′-cyclic monophosphate in adrenoceptor-mediated control of 3H-noradrenaline secretion in guinea-pig ileum myenteric nerve terminals}, author={Pēteris Alberts and Viviann R. {\"{O}gren and {\AA}ke I. Sellstr{\"{o}m}, journal={Naunyn-Schmiedeberg's Archives of Pharmacology}, year={1985}, volume={330}, pages={114-120} }