Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae.

@article{Karpova2000RoleOA,
  title={Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae.},
  author={Tatiana S. Karpova and Samara L Reck-Peterson and N. Barry Elkind and Mark S. Mooseker and Peter Jay Novick and John A. Cooper},
  journal={Molecular biology of the cell},
  year={2000},
  volume={11 5},
  pages={
          1727-37
        }
}
We examined the role of the actin cytoskeleton in secretion in Saccharomyces cerevisiae with the use of several quantitative assays, including time-lapse video microscopy of cell surface growth in individual living cells. In latrunculin, which depolymerizes filamentous actin, cell surface growth was completely depolarized but still occurred, albeit at a reduced level. Thus, filamentous actin is necessary for polarized secretion but not for secretion per se. Consistent with this conclusion… CONTINUE READING
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