Role of acrolein in cyclophosphamide teratogenicity in rat embryos in vitro.

@article{Mirkes1984RoleOA,
  title={Role of acrolein in cyclophosphamide teratogenicity in rat embryos in vitro.},
  author={Philip E. Mirkes and John Greenaway and John G. Rogers and Robert B. Brundrett},
  journal={Toxicology and applied pharmacology},
  year={1984},
  volume={72 2},
  pages={281-91}
}
To elucidate the role of acrolein in cyclophosphamide (CP) teratogenesis, we used the dechloro derivative of cyclophosphamide (D-CP). After activation, D-CP spontaneously breaks down to yield acrolein and dechlorophosphoramide mustard (D-PM), the nonalkylating derivative of phosphoramide mustard. At concentrations ranging from 6.25 to 50 micrograms/ml (33 to 262 microM), D-CP produced concentration-dependent cell death, growth retardation, and malformations in rat embryos cultured in vitro from… CONTINUE READING