Role of Tyrosine Phosphorylation of HS1 in B Cell Antigen Receptor-mediated Apoptosis

@article{Yamanashi1997RoleOT,
  title={Role of Tyrosine Phosphorylation of HS1 in B Cell Antigen Receptor-mediated Apoptosis},
  author={Yuji Yamanashi and Takahiro Fukuda and Hirofumi Nishizumi and Tetsuya Inazu and Ken-ichi Higashi and Daisuke Kitamura and Takaomi Ishida and Hirohei Yamamura and Takeshi Watanabe and Tadashi Yamamoto},
  journal={The Journal of Experimental Medicine},
  year={1997},
  volume={185},
  pages={1387 - 1392}
}
The 75-kD HS1 protein is highly tyrosine-phosphorylated during B cell antigen receptor (BCR)-mediated signaling. Owing to low expression of HS1, WEHI-231-derived M1 cells, unlike the parental cells, are insensitive to BCR-mediated apoptosis. Here, we show that BCR-associated tyrosine kinases Lyn and Syk synergistically phosphorylate HS1, and that Tyr-378 and Tyr-397 of HS1 are the critical residues for its BCR-induced phosphorylation. In addition, unlike wild-type HS1, a mutant HS1 carrying the… CONTINUE READING