Role of RpoS and MutS in phase variation of Pseudomonas sp. PCL1171.

@article{Broek2005RoleOR,
  title={Role of RpoS and MutS in phase variation of Pseudomonas sp. PCL1171.},
  author={Daan A. van den Broek and Thomas F. C. Chin-A-Woeng and Guido Vincent Bloemberg and Ben Lugtenberg},
  journal={Microbiology},
  year={2005},
  volume={151 Pt 5},
  pages={
          1403-8
        }
}
Pseudomonas sp. strain PCL1171 undergoes reversible colony phase variation between opaque phase I and translucent phase II colonies, which is dependent on spontaneous mutations in the regulatory genes gacA and gacS. Mutation of the mutS gene and constitutive expression of rpoS increases the frequency at which gac mutants appear 1000- and 10-fold, respectively. Experiments were designed to study the relationship between gacS, rpoS and mutS. These studies showed that (i) a functional gac system… CONTINUE READING

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Leiden Genome Technology Centre for the opportunity to use their equipment, and G. Girard (Institute of Biology, Universiteit Leiden) for supplying us with plasmids pMP7420 and pMP7418

  • W. Achouak, S. Conrod, V. Cohen, T. Heulin
  • Phenotypic
  • 2004
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