Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid.

@article{Betteridge2004RoleOR,
  title={Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid.},
  author={Thu Betteridge and Jing Yang and Alexandre Pittard and Judy Praszkier},
  journal={Journal of bacteriology},
  year={2004},
  volume={186 12},
  pages={3785-93}
}
The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5'-TCTTAA-3… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 60 references

Molecular cloning: a laboratory manual, 3rd ed

  • J. Sambrook, D. W. Russell
  • 2001
2 Excerpts

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