Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity

@article{Pigott2007RoleOR,
  title={Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity},
  author={Craig Pigott and David J. Ellar},
  journal={Microbiology and Molecular Biology Reviews},
  year={2007},
  volume={71},
  pages={255 - 281}
}
SUMMARY Bacillus thuringiensis produces crystalline protein inclusions with insecticidal or nematocidal properties. These crystal (Cry) proteins determine a particular strain's toxicity profile. Transgenic crops expressing one or more recombinant Cry toxins have become agriculturally important. Individual Cry toxins are usually toxic to only a few species within an order, and receptors on midgut epithelial cells have been shown to be critical determinants of Cry specificity. The best… 

Multiple receptors as targets of Cry toxins in mosquitoes.

TLDR
An overview of the understanding of the interactions between Cry toxin and multiple receptors in mosquitoes, in particular Aedes aegypti is provided and the manner by which the receptors were identified and characterized is reviewed, with a focus on three proteins, cadherin, alkaline phosphatase, and aminopeptidase-N.

Identification and Characterization of Receptors for Insecticidal Toxins from Bacillus thuringiensis

TLDR
This chapter discusses about the different methodologies used to identify and to study different insect gut proteins that have been shown to bind Cry toxins and that may be involved in Cry toxin action.

The Cytocidal Spectrum of Bacillus thuringiensis Toxins: From Insects to Human Cancer Cells

TLDR
It was recently demonstrated that SLP produced by Bt could interact with membrane receptors of insect and human cancer cells to kill them, and Cyt toxins have a structure that is mostly unrelated to Cry toxins; thereby, other mechanisms of action have been reported to them.

Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection.

TLDR
Activity of Cry1AMod toxins, which are able to form toxin oligomers in the absence of receptors, against different resistant populations, supports the hypothesis that toxin oligomerization is a limiting step in the Cry insecticidal activity.

Bacillus thuringiensis: A story of a successful bioinsecticide.

Cytocidal activity of Cry41Aa, an anticancer toxin from Bacillus thuringiensis

TLDR
In an attempt to better understand the mechanism of action of Cry41Aa against cancer cells, evolved resistance in HepG2 through repeated exposure to increasing doses of the toxin and showed that in response to the toxin p38, but not ERK1/2, is activated and in a dose dependent manner.

Cell lines as models for the study of Cry toxins from Bacillus thuringiensis.

Promise of Bacillus thuringiensis Crystal Proteins as Anthelmintics

TLDR
Cry proteins offer excellent combinatorial therapeutic properties with nicotinic acetylcholine receptor agonists – one of two classes of compounds approved by the World Health Organization for treatment of intestinal roundworms in humans.
...

References

SHOWING 1-10 OF 216 REFERENCES

Insecticidal crystal proteins of Bacillus thuringiensis.

TLDR
Studies on the biochemical mechanisms of toxicity suggest that B. thuringiensis crystal proteins induce the formation of pores in membranes of susceptible cells, and these approaches are potentially powerful strategies for the protection of agriculturally important crops against insect damage.

Insecticidal crystal proteins of Bacillus thuringiensis

TLDR
Studies on the biochemical mechanisms of toxicity suggest that B. thuringiensis crystal proteins induce the formation of pores in membranes of susceptible cells, and these approaches are potentially powerful strategies for the protection of agriculturally important crops against insect damage.

Cry toxin mode of action in susceptible and resistant Heliothis virescens larvae.

Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation.

TLDR
Comparison with the structure of CryIIIA, a coleopteran-specific toxin, shows that although the fold of these two proteins is similar, there are significant structural differences within domain II, which supports the conclusions from genetic studies that domain II is involved in recognition and binding to cell surface receptors.

Bacillus thuringiensis crystal proteins that target nematodes

TLDR
Toxicity in nematodes correlates with damage to the intestine, consistent with the mechanism of crystal toxin action in insects, and structure–function analyses indicate that one novel nematicidal crystal protein can be engineered to a small 43-kDa active core.

Identification of Bombyx mori midgut receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin.

TLDR
Results indicated that BtR175 was the receptor protein for the insecticidal toxin, which could not be explained only in term of the existence of its binding protein.

Aggregation of Bacillus thuringiensisCry1A Toxins upon Binding to Target Insect Larval Midgut Vesicles

TLDR
There was a greater extent of oligomerization of the Cry1Ac toxin with vesicles from theHeliothis virescens midgut than with those from theM.

Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family proteins and their functional domains

  • A. Bravo
  • Biology
    Journal of bacteriology
  • 1997
TLDR
Insecticidal crystal proteins (ICPs) from Bacillus thuringiensis have been used as biopesticides for the last 35 years and a novel nomenclature has been proposed based exclusively on amino acid identity.
...