Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae.

  title={Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae.},
  author={Susan M Miller and Boris Magasanik},
  journal={Journal of bacteriology},
  volume={172 9},
We cloned GDH2, the gene that encodes the NAD-linked glutamate dehydrogenase in the yeast Saccharomyces cerevisiae, by purifying the enzyme, making polyclonal antibodies to it, and using the antibodies to screen a lambda gt11 yeast genomic library. A yeast strain with a deletion-disruption allele of GDH2 which replaced the wild-type gene grew very poorly with glutamate as a nitrogen source, but growth improved significantly when the strain was also provided with adenine or other nitrogenous… CONTINUE READING
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Isolation and characterization of a Saccharomyces cerevisiae mutant with impaired glutamate synthase activity

  • M. Grenson, E. Dubois, M. Piotrowska, R. Driflien, M. Aigle
  • J . Bacteriol .
  • 1989

Constructing and screening cDNA libraries in AgtlO and Xgtll , p . 49 - 88

  • D. M. Glover
  • DNA cloning : a practical approach
  • 1985

Nitrogen catab - olite repression in yeasts and filamentous fungi

  • R. M. Wohlheuter, H. Schutt, H. Holzer
  • Adv . Microb . Physiol .
  • 1985

A catabolic L - serine ( L - threonine ) deaminase occurs in yeast

  • G. S. Roeder, G. R. Fink
  • Eur . J . Biochem .
  • 1982

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