Role of Mutation in Pseudomonas aeruginosa Biofilm Development

@article{Conibear2009RoleOM,
  title={Role of Mutation in Pseudomonas aeruginosa Biofilm Development},
  author={T. Conibear and Samuel Collins and J. Webb},
  journal={PLoS ONE},
  year={2009},
  volume={4}
}
The survival of bacteria in nature is greatly enhanced by their ability to grow within surface-associated communities called biofilms. Commonly, biofilms generate proliferations of bacterial cells, called microcolonies, which are highly recalcitrant, 3-dimensional foci of bacterial growth. Microcolony growth is initiated by only a subpopulation of bacteria within biofilms, but processes responsible for this differentiation remain poorly understood. Under conditions of crowding and intense… Expand
Antibiotic resistance of bacterial biofilms.
TLDR
Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy and a promising strategy may be the use of enzymes that can dissolve the biofilm matrix as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics. Expand
Environmental cues and genes involved in establishment of the superinfective Pf4 phage of Pseudomonas aeruginosa
TLDR
The results suggest that the accumulation of RONS by the biofilm may result in DNA lesions in the Pf4phage, leading to the formation of SI phage, which subsequently selects for morphotypic variants, such as SCVs. Expand
Biofilm: A Hotspot for Emerging Bacterial Genotypes.
TLDR
This review summarizes the present knowledge within this important area of research on bacterial evolution in biofilms and indicates that there is growing evidence that the biofilm lifestyle may be more mutagenic than planktonic growth. Expand
Effect of long-term starvation in salty microcosm on biofilm formation and motility in Pseudomonas aeruginosa
TLDR
A comparison of the biofilm produced by the wild-type strain PA14 and the transposon insertion mutant for speD gene suggested that spermidine has a potential role in the adaptive response in P. aeruginosa incubated in long-term stress conditions. Expand
Fitness Landscape of Antibiotic Tolerance in Pseudomonas aeruginosa Biofilms
TLDR
This study used genome-wide functional profiling of a complex, heterogeneous mutant population of Pseudomonas aeruginosa MPAO1 in biofilm and planktonic growth conditions with and without tobramycin to systematically quantify the contribution of each locus to antibiotic tolerance under these two states. Expand
Planktonic Aggregates of Staphylococcus aureus Protect against Common Antibiotics
TLDR
The data indicate that the properties of cells in aggregates differ in some aspects from those in biofilms, and speculate that in aggregate communities S. aureus increases its tolerance to hazardous environments and that the combination of a biofilm-like environment with mobility has substantial practical and clinical importance. Expand
Bacterial Biofilm and Antibiotic Resistance
Biofilm is a structural and functional unit of microbial consortia encased in self-produced polymeric substances including protein, polysaccharide and e-DNA. Biofilm formation by bacteria representsExpand
Involvement of Stress-Related Genes polB and PA14_46880 in Biofilm Formation of Pseudomonas aeruginosa
TLDR
Results indicate that inactivation of polB and PA14_46880 may inhibit transition of P. aeruginosa from a more acute infection lifestyle to the biofilm phenotype, and future investigation of these genes may lead to a better understanding of the mechanisms behind biofilm formation and chronic biofilm infections. Expand
Antimicrobial susceptibility testing in biofilm-growing bacteria.
  • M. Maciá, E. Rojo-Molinero, A. Oliver
  • Biology, Medicine
  • Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2014
TLDR
Standardization of the procedures, parameters and breakpoints, by official agencies, is needed before they are implemented in clinical microbiology laboratories for routine susceptibility testing, to obtain a deeper understanding of biofilm resistance mechanisms. Expand
Evolution and Adaptation in Pseudomonas aeruginosa Biofilms Driven by Mismatch Repair System-Deficient Mutators
TLDR
Through competition experiments, it is demonstrated for the first time that P. aeruginosa MRS-deficient mutators had enhanced adaptability over wild-type strains when grown in structured biofilms but not as planktonic cells, providing further evidence for mutator-driven adaptive evolution in the biofilm mode of growth. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 55 REFERENCES
Cell Death in Pseudomonas aeruginosa Biofilm Development
TLDR
It is proposed that prophage-mediated cell death is an important mechanism of differentiation inside microcolonies that facilitates dispersal of a subpopulation of surviving cells in biofilms of P. aeruginosa. Expand
Bacteriophage and Phenotypic Variation in Pseudomonas aeruginosa Biofilm Development
TLDR
Pf4 can mediate phenotypic variation in P. aeruginosa biofilms and causes the emergence of SCVs within the culture, which exhibited enhanced attachment, accelerated biofilm development, and large regions of dead and lysed cells inside microcolonies in a manner identical to that ofSCVs obtained from biofilmms. Expand
Increased mutability of Pseudomonas aeruginosa in biofilms.
TLDR
Down-regulation of antioxidant enzymes in P. aeruginosa biofilms may enhance the rate of mutagenic events due to the accumulation of DNA damage and provide a further source of antibiotic-resistant mutants in the CF lung. Expand
Involvement of bacterial migration in the development of complex multicellular structures in Pseudomonas aeruginosa biofilms
TLDR
It is shown that mushroom‐shaped multicellular structures in P. aeruginosa biofilms can form in a sequential process involving a non‐motile bacterial subpopulation and a migrating bacterial sub population. Expand
Differentiation and Distribution of Colistin- and Sodium Dodecyl Sulfate-Tolerant Cells in Pseudomonas aeruginosa Biofilms
TLDR
Increased antibiotic tolerance in biofilms may be a consequence of differentiation into distinct subpopulations with different phenotypic properties, as suggested by mutations in genes interfering with lipopolysaccharide modification. Expand
Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm.
TLDR
The results demonstrate that P. aeruginosa displays multiple phenotypes during biofilm development and that knowledge of stage-specific physiology may be important in detecting and controlling biofilm growth. Expand
Endogenous oxidative stress produces diversity and adaptability in biofilm communities
TLDR
It is hypothesized that the mutations produced could promote the adaptation of biofilm communities to changing conditions in addition to generating diversity, and it was found that the oxidative stress-break repair mechanism increased the emergence of antibiotic-resistant bacteria. Expand
Development and dynamics of Pseudomonas sp. biofilms.
TLDR
Experiments involving a nonflagellated P. putida OUS82 mutant suggested that the movements between and inside microcolonies were flagellum driven, suggesting that biofilm bacteria are in a physiological state different from planktonic bacteria. Expand
Phenotypic differentiation and seeding dispersal in non-mucoid and mucoid Pseudomonas aeruginosa biofilms.
TLDR
Using the flow cell culturing method, the effects of quorum sensing (QS) and rhamnolipid production (factors previously identified as important in determining biofilm structure) on seeding dispersal using knockout mutants isogenic with PAO1 was investigated. Expand
Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation
TLDR
It is found that antibiotic-resistant phenotypic variants of P. aeruginosa with enhanced ability to form biofilms arise at high frequency both in vitro and in the lungs of CF patients. Expand
...
1
2
3
4
5
...