Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing

@article{Cao2002RoleOH,
  title={Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing},
  author={Ru Cao and Liangjun Wang and Hengbin Wang and Li Xia and Hediye Erdjument-Bromage and Paul Tempst and Richard S. Jones and Yi Zhang},
  journal={Science},
  year={2002},
  volume={298},
  pages={1039 - 1043}
}
Polycomb group (PcG) proteins play important roles in maintaining the silent state of HOX genes. Recent studies have implicated histone methylation in long-term gene silencing. However, a connection between PcG-mediated gene silencing and histone methylation has not been established. Here we report the purification and characterization of an EED-EZH2 complex, the human counterpart of theDrosophila ESC-E(Z) complex. We demonstrate that the complex specifically methylates nucleosomal histone H3… Expand
Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27.
TLDR
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Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes
TLDR
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TLDR
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Substrate Preferences of the EZH2 Histone Methyltransferase Complex*
TLDR
The hypothesis that linker histone H1 and chromatin structure are important factors in determining the substrate preference of the EZH2 histone methyltransferase complex is supported, which suggests that Embryonic Ectoderm Development EED isoforms may affect substrate specificity. Expand
Molecular and functional mapping of EED motifs required for PRC2-dependent histone methylation.
TLDR
It is demonstrated that the core WD-40 motifs and the histone-binding region of EED alone are sufficient for the generation of all three marks, demonstrating that EED isoforms do not control the number of methyl groups added to H3K27. Expand
Polycomb Complexes and the Propagation of the Methylation Mark at the Drosophila Ubx Gene*
TLDR
It is proposed that the Polycomb chromodomain is required for the looping mechanism that spreads methylation over a broad domain, which in turn is needed for the stability of thePolycomb group protein complex. Expand
Dense Chromatin Activates Polycomb Repressive Complex 2 to Regulate H3 Lysine 27 Methylation
TLDR
It is proposed that PRC2 can sense the chromatin environment to exert its role in the maintenance of transcriptional states, and activity is regulated by the density of its substrate nucleosome arrays. Expand
Role of hPHF1 in H3K27 Methylation and Hox Gene Silencing
TLDR
The results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing. Expand
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