Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.

@article{Skarphol1997RoleOH,
  title={Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.},
  author={K Skarphol and Jill K. Waukau and Steve A Forst},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 4},
  pages={1413-6}
}
EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate… CONTINUE READING
Highly Cited
This paper has 35 citations. REVIEW CITATIONS
15 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

Similar Papers

Loading similar papers…