Role of Hemichrome Binding to Erythrocyte Membrane in the Generation of Band-3 Alterations in P-Thalassemia Intermedia Erythrocytes

Abstract

Nine splenectomized, hematologically well-compensated pthalassemia intermedia patients randomly chosen from a pool of 60 similar patients were studied. Membrane proteins solubilized with nondenaturing detergent &E8 were gel filtered on Sepharose CL-GB (Pharmacia Fine Chemicals, Uppsala, Sweden). Fractions containing higher than 4,000-kD molecular-weight aggregates were isolated and analyzed. Four patients had remarkably increased amounts of membrane-bound hemichromes and Igs. In those patients, band 3 underwent oxidative modifications such as aggregation and a decrease in sulfhydryl groups. The other five patients had low amounts of membrane-bound hemichromes and less modifications of band 3. The same band-3 modifications could be reproduced by challenging normal membranes

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Cite this paper

@inproceedings{Mannu2002RoleOH, title={Role of Hemichrome Binding to Erythrocyte Membrane in the Generation of Band-3 Alterations in P-Thalassemia Intermedia Erythrocytes}, author={Franca Mannu and Paolo Arese and Maria Domenica Cappellini and Gernino Fiorelli and Marina Cappadoro and Giuliana Giribaldi and Francesco M Turrini}, year={2002} }