Role of GATA-3 in IL-5 Gene Transcription by CD4+ T Cells of Asthmatic Patients

@article{Kaminuma2005RoleOG,
  title={Role of GATA-3 in IL-5 Gene Transcription by CD4+ T Cells of Asthmatic Patients},
  author={Osamu Kaminuma and Akio Mori and Noriko Kitamura and Tomomi Hashimoto and Fujiko Kitamura and Shigeko Inokuma and Shoichiro Miyatake},
  journal={International Archives of Allergy and Immunology},
  year={2005},
  volume={137},
  pages={55 - 59}
}
Background: Helper T cells and T cell cytokines play central roles in allergic disorders including bronchial asthma. We reported enhanced IL-5 production by peripheral blood T cells of asthmatic patients. A transcription factor, GATA-3, has been implicated in IL-5 gene expression. This study was undertaken to clarify the role of GATA-3 in the upregulation of IL-5 synthesis in asthmatic patients. Method: Peripheral CD4+ T cells were transfected with an IL-5 promoter reporter construct as well as… 

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References

SHOWING 1-10 OF 35 REFERENCES
Transcription Factor GATA-3 Is Differentially Expressed in Murine Th1 and Th2 Cells and Controls Th2-specific Expression of the Interleukin-5 Gene*
TLDR
It is demonstrated that GATA-3 is critical for expression of the IL-5 gene in bona fide Th2 cells and has therapeutic potential in the treatment of asthma and other hypereosinophilic diseases.
Differential responsiveness of the IL-5 and IL-4 genes to transcription factor GATA-3.
TLDR
It is shown that ectopic expression of GATA-3 is sufficient to drive IL-5 but not IL-4 gene expression, and in Th2 cells, antisense Gata-3 RNA inhibits IL- 5 but notIL-4 promoter activation.
Regulation of the Human Interleukin-5 Promoter by Ets Transcription Factors
TLDR
The synergism of GATA3 with either Ets1 or Ets2 may play an important role in calcium- or Tax1-dependent regulation of IL-5 expression in Th2 cells or in HTLV-I transformed adult T-cell leukemia cells, respectively.
GATA-3 Has Dual Regulatory Functions in Human Interleukin-5 Transcription*
TLDR
It is shown here that GATA-3 has another function in controlling IL-5 transcription that supports the observed unique biological function of this cytokine.
A nuclear factor of activated T cell-like transcription factor in mast cells is involved in IL-5 gene regulation after IgE plus antigen stimulation.
TLDR
It is reported that an inducible activity in the region between -177 and -80, and a constitutive activity between -80 and -70, in the promoter of the human gene, are both necessary for the allergically triggered activation of human IL-5.
Friend of GATA Is Expressed in Naive Th Cells and Functions As a Repressor of GATA-3-Mediated Th2 Cell Development1
TLDR
Serial deletion mutation analysis indicated that the N-terminal region, but not the consensus C-Terminal binding protein-binding motif, of FOG is critical for the effects of GATA-3 in naive T cells.
Upregulation of the transcription factor GATA-3 in upper airway mucosa after in vivo and in vitro allergen challenge.
TLDR
The expression of the transcription factor GATA-3 was increased after allergen challenge, and this was evident in the absence of de novo inflammatory cell recruitment, which may be a potential target in the treatment of allergic diseases, such as rhinitis.
Transcriptional regulation of the IL‐5 gene in peripheral T cells of asthmatic patients
TLDR
Data suggests that the composite NFAT/AP‐1 binding element plays a crucial role in IL‐5 synthesis by peripheral T cells of asthmatic patients.
...
...