Role of DNA topology in Mu transposition: mechanism of sensing the relative orientation of two DNA segments.

@article{Craigie1986RoleOD,
  title={Role of DNA topology in Mu transposition: mechanism of sensing the relative orientation of two DNA segments.},
  author={Robert Craigie and Kiyoshi Mizuuchi},
  journal={Cell},
  year={1986},
  volume={45 6},
  pages={793-800}
}
DNA strand transfer at the initiation of Mu transposition normally requires a negatively supercoiled transposon donor molecule, containing both ends of Mu in inverted repeat orientation. We propose that the specific relative orientation of the Mu ends is needed only to energetically favor a particular configuration that the ends must adopt in a synaptic complex. The model was tested by constructing special donor DNA substrates that, because of their catenation or knotting, energetically favor… CONTINUE READING

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