Role of DNA replication in vaccinia virus gene expression: a naked template is required for transcription of three late trans-activator genes.

@article{Keck1990RoleOD,
  title={Role of DNA replication in vaccinia virus gene expression: a naked template is required for transcription of three late trans-activator genes.},
  author={Julia Keck and Carl J. Baldick and Bernard Moss},
  journal={Cell},
  year={1990},
  volume={61 5},
  pages={801-9}
}
The DNA replication requirement for vaccinia virus late gene expression was mimicked by transfecting a late promoter-controlled reporter gene into infected cells in the presence of a DNA synthesis inhibitor. This late promoter activation block was overcome by cotransfecting either naked linear vaccinia virion DNA or three cloned viral genes encoding trans-activator polypeptides of 17, 26, and 30 kd. These newly identified trans-activator genes were independently transcribed only from replicated… CONTINUE READING

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