Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes

@article{Ji2016RoleOC,
  title={Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes},
  author={Hao Ji and Bin Zhuge and Hong Zong and Xinyao Lu and Hui-ying Fang and Jian Zhuge},
  journal={Current Microbiology},
  year={2016},
  volume={73},
  pages={827-833}
}
Candida glycerinogenes, the glycerol producer with excellent multi-stress tolerances, is considered to be a potential biotechnological host used in the production of glycerol and its derivatives under extreme fermentation conditions. [...] Key Result Pseudohyphae generation of the CgHOG1 mutant under non-inducing condition indicated a repressor role in morphological transitions.Expand
CgHog1-Mediated CgRds2 Phosphorylation Alters Glycerophospholipid Composition To Coordinate Osmotic Stress in Candida glabrata
TLDR
It is demonstrated that CgHog1-mediated CgRds2 phosphorylation enhanced glycerophospholipid composition and membrane integrity to resist osmotic stress in C. glabrata. Expand
Functional and expression studies of two novel STL1 genes of the osmotolerant and glycerol utilization yeast Candida glycerinogenes.
TLDR
Two STL1 homologues CgSTL1 and Cg STL2 encoding the putative glycerol transporters were identified, and their products were found to be localized to plasma membranes by tagging GFP protein. Expand
γ-aminobutyric acid accumulation enhances the cell growth of Candida glycerinogenes  under hyperosmotic conditions.
TLDR
It is demonstrated that GABA accumulation plays an important role in osmotic stress resistance of the unconventional yeast C. glycerinogenes and improving intracellular GABA accumulation, either through exogenous application or cellular synthesis, is available for improving the tolerance to hyperosmotic stress. Expand
Gene expression profiles of Candida glycerinogenes under combined heat and high-glucose stresses.
TLDR
The wider responsive gene spectrum and the activated expression of ribosomal function related genes might be key and prerequisite factors for the excellent tolerance to the combined stress of C. glycerinogenes. Expand
Improving the productivity of Candida glycerinogenes in the fermentation of ethanol from non‐detoxified sugarcane bagasse hydrolysate by a hexose transporter mutant
TLDR
The results provide a promising strategy for constructing efficient strains for ethanol production by identifying the hexose transporter in this yeast that makes a high contribution to glucose consumption, and by adding additional copies of this transporter and enhancing its membrane localization stability (MLS). Expand
Identification and characterization from Candida glycerinogenes of hexose transporters having high efficiency at high glucose concentrations
TLDR
Results indicate that CgHxt4 plays an important role in the fermentation process as a hexose transporter with strong transport activity and efficient expression regulation at high glucose concentrations. Expand
Establishment of a transient CRISPR-Cas9 genome editing system in Candida glycerinogenes for co-production of ethanol and xylonic acid.
TLDR
Results indicated that the CRSIPR-Cas9 system developed here can facilitate the study of gene functions and metabolic pathways in C. glycerinogenes. Expand
Transcription factor Hap5 induces gsh2 expression to enhance 2-phenylethanol tolerance and production in an industrial yeast Candida glycerinogenes
TLDR
It is discovered that Hap5 is an essential regulator to 2- PE resistance, and its induction by 2-PE stress occurs at the post-transcriptional level, rather than at the transcriptional level. Expand
Selection and application of novel high temperature inducible promoters in the tolerant yeast Candida glycerinogenes.
TLDR
QRT-PCR results showed a potential to high temperature fermentation on the stress tolerant C. glycerinogenes and some of novel high temperature inducible promoters. Expand
Identification and application of novel low pH-inducible promoters for lactic acid production in the tolerant yeast Candida glycerinogenes.
TLDR
The results presented here showed a potential to produce organic acid economically at low pH by the stress tolerant C. glycerinogenes and the novel low-pH inducible promoter PCggmt1. Expand
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Identification of a Novel HOG1 Homologue from an Industrial Glycerol Producer Candida glycerinogenes
TLDR
To understand the osmo-adaptation mechanism in C. glycerinogenes, the mitogen-activated protein kinase HOG1 gene (CgHOG1), which plays an essential role in the yeast hyperosmotic response, was isolated by degenerate PCR and SEFA-Formed Adaptor PCR and transformed in Saccharomyces cerevisiaehog1Δ null mutant. Expand
The MAP kinase Hog1p differentially regulates stress-induced production and accumulation of glycerol and D-arabitol in Candida albicans.
TLDR
The ability to grow and produce glycerol when exposed to osmotic or citric acid stresses, and to produce d-arabitol when exposure to oxidative stress, was partially dependent on Hog1p, but the ability to producing d-ArabitolWhen exposed to temperature stress was Hog1P independent, implying that multiple pathways regulate glycerl and d-ARabitol synthesis in C. albicans. Expand
Glycerol formation during wine fermentation is mainly linked to Gpd1p and is only partially controlled by the HOG pathway
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Data show that Gpd1p plays a major role in glycerol formation, particularly during the first few hours of exposure to high sugar concentration, and that GPD2 is only of little significance in anaerobic fermentation by wine yeast. Expand
Hog1p mitogen-activated protein kinase determines acetic acid resistance in Saccharomyces cerevisiae.
TLDR
In low-pH (pH 4.5) cultures, the acetate-activated Hog1p, although conferring acetate resistance, does not generate the GPD1 gene or intracellular glycerol inductions that are hallmarks of activation of the HOG pathway by hyperosmotic stress. Expand
Cloning and characterization of a NAD+-dependent glycerol-3-phosphate dehydrogenase gene from Candida glycerinogenes, an industrial glycerol producer.
TLDR
Cloned and characterized a 4900-bp genomic fragment containing the CgGPD gene encoding a glycerol-3-phosphate dehydrogenase homologous to GPD genes in other yeasts using degenerate primers in conjunction with inverse PCR, suggesting that it is a functional GPD protein. Expand
Glycerol production by a novel osmotolerant yeast Candida glycerinogenes
TLDR
Candida glycerinogenes, an osmotolerant yeast isolated from a natural sample in an environment of high osmotic pressure, had a modest sugar-tolerance and an extremely high glycerol productivity and was obtained by using a 30-l agitated fermentor under optimal fermentation conditions. Expand
The Cek1 and Hog1 Mitogen-Activated Protein Kinases Play Complementary Roles in Cell Wall Biogenesis and Chlamydospore Formation in the Fungal Pathogen Candida albicans
TLDR
It is shown here that repression of filamentous growth occurs both under serum limitation and under other partially inducing conditions, such as low temperature, low pH, or nitrogen starvation. Expand
Saccharomyces cerevisiae glycerol/H+ symporter Stl1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is given
TLDR
Any S. cerevisiae strain already in use can become more resistant to cold/freeze-thaw stress just by simply adding glycerol to the broth, which will induce the transporter Stl1p and consequently cell survival at cold/near-freeze and freeze temperatures. Expand
Metabolic regulation rather than de novo enzyme synthesis dominates the osmo‐adaptation of yeast
TLDR
The regulation of the glycerol pathway was analysed in aerobic, glucose‐limited cultures upon addition of 1 M of sorbitol, leading to a hyperosmotic shock and demonstrates that the novel metabolic mechanism is not just a secondary rescue mechanism, but the most important mechanism to regulate the glycersol flux under physiological conditions. Expand
Response to Hyperosmotic Stress
TLDR
The current understanding of both the upstream signaling mechanism and the downstream adaptive responses to hyperosmotic stress in yeast are summarized. Expand
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