Corpus ID: 81452886

Role of CSL glycoprotein in infectivity and neutralization of Cryptosporidium parvum sporozoites

  title={Role of CSL glycoprotein in infectivity and neutralization of Cryptosporidium parvum sporozoites},
  author={R. Langer},
Cryptosporidiosis, caused by the apicomplexan parasite Cryptosporidium parvum, has become a well recognized diarrheal disease of immunodeficient humans and other mammals throughout the world. Specific therapy and immunoprophylaxis are currently unavailable, but passive immunization with C. /^arvum-specific monoclonal antibodies (mAbs) has demonstrated efficacy in immunocompromised hosts. In particular, mAbs eliciting the circumsporozoite precipitate (CSP)-like reaction protected against C… Expand

Tables from this paper


Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites.
Findings indicate that CSL has a functional role in sporozoite infectivity and is a candidate molecular target for passive or active immunization against cryptosporidiosis. Expand
Neutralizing Monoclonal Antibody Protects Against Cryptosporidium parvum Infection by Inhibiting Sporozoite Attachment and Invasion
Because CSL is mechanistically involved in the CSP-like reaction, it was purified for further study of its potential role in the infection process, and sporozoite attachment and invasion assays were performed in vitro, allowing examination of sporozoites-host cell interactions not readily studied in vivo. Expand
Cryptosporidium parvum merozoites share neutralization-sensitive epitopes with sporozoites.
It is shown that C. parvum merozoites share neutralization-sensitive epitopes with sporozoites, and infection by sporozoite infectivity can also be neutralized by surface-reactive mAb. Expand
Neutralization-sensitive epitopes are exposed on the surface of infectious Cryptosporidium parvum sporozoites.
The results indicate that two different molecules capable of inducing neutralizing antibody are exposed on the surface of C. parvum sporozoites. Expand
Infectivity and neutralization of Cryptosporidium parvum sporozoites
When isolated sporozoites were incubated with heat-inactivated immune bovine serum, 25 times the 50% infective dose for 7-day-old mice was completely neutralized and sporozoite infectivity in vivo by direct intraintestinal injection of isolated sporzoites in 7- day-old BALB/c mice. Expand
Bovine antibody against Cryptosporidium parvum elicits a circumsporozoite precipitate-like reaction and has immunotherapeutic effect against persistent cryptosporidiosis in SCID mice
Hyperimmune bovine antibody prepared against C. parvum may provide a first-generation therapy for control of cryptosporidiosis, and the defined antigens can be evaluated as subunit immunogens to produce better-characterized polyclonal antibody for control. Expand
Characterization of a > 900,000-M(r) Cryptosporidium parvum sporozoite glycoprotein recognized by protective hyperimmune bovine colostral immunoglobulin
The identification and initial characterization of a > 900,000-M(r) Cryptosporodium sporozoite glycoprotein (GP900) that is a prominent antigen recognized by protective hyperimmune bovine colostral immunoglobulin is reported, indicating that the molecule is highly immunogenic in mice as well as in cows. Expand
Cryptosporidium parvum development in the BS-C-1 cell line.
This is the first report of C. parvum completing its life cycle in BS-C-1, an African green monkey kidney cell line, and both sodium hypochlorite-stimulated oocysts and purified sporozoites were able to initiate infection that led to completion of the whole life cycle. Expand
Antigens of Cryptosporidium sporozoites recognized by immune sera of infected animals and humans.
Serum reactivity to the 20-kDa antigen appears to be a good indicator of exposure to Cryptosporidium. Expand
Immunotherapy of cryptosporidiosis in immunodeficient animal models.
The results obtained indicate that SCID foals are a useful large animal model of clinical disease associated with persistent C. parvum infection, and that nude mice are a convenient animal model for testing therapeutic potential of antibodies in persistent cryptosporidial infection. Expand