Corpus ID: 28240346

Role of CD44 in the development of natural killer cells from precursors in long-term cultures of mouse bone marrow.

  title={Role of CD44 in the development of natural killer cells from precursors in long-term cultures of mouse bone marrow.},
  author={D. Delfino and K. Patrene and A. Deleo and R. Deleo and R. Herberman and S. Boggs},
  journal={Journal of immunology},
  volume={152 11},
The role of the adhesion molecule CD44 in the development of NK cells was analyzed in a mouse long-term bone marrow culture system. After 4 wk of culture (day 0), recombinant human IL-2 was added and 13 days later the cells generated were shown to have substantial cytotoxic activity against YAC-1 and to be enriched for NK cells, as assessed for NK-1.1 phenotype by flow cytometric analysis. Physical separation between stroma and precursors partially inhibited proliferation and, consequently, a… Expand
Characterization of the stage in natural killer cell development in 14.5-day mouse fetal liver using adult bone marrow stroma.
Together, these data suggest that the NK lineage cells in FL are primarily in early stages of development, are highly proliferative, respond to early acting cytokines and express stem cell markers. Expand
Expression of murine CD34 by fetal liver NK cell progenitors.
It is suggested that both CD34+ and CD34- subpopulations of FL can differentiate into NK cells when cocultured for 13 days with irradiated NK-LTBMC stroma and rhIL-2, and thatCD34+ progenitors differentiate toCD34- precursors, which in turn differentiate to CD 34- mature NK cells. Expand
CD44 and TGFbeta1 synergise to induce expression of a functional NADPH oxidase in promyelocytic cells.
It is concluded that cell matrix factors act cooperatively with cytokines to induce the expression of the components of the NADPH-oxidase in monocytic progenitor cells. Expand
CD44v10 in Hematopoiesis and Stem Cell Mobilization
  • M. Zoeller
  • Biology, Medicine
  • Leukemia & lymphoma
  • 2000
Treatment with anti-CD44v10 revealed promising results concerning the recovery of multilineage colony forming units in the spleen and the peripheral blood, and further evidence is provided for anti- CD 44v10 as a means to mobilize hematopoietic progenitor cells. Expand
Effects of anti-CD 44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines
Acute myeloid leukemia (AML) is a heterogeneous leukemia characterized by the blockage of myeloid differentiation at different stages, which define distinct AML subtypes. We have recently reportedExpand
Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines.
It is demonstrated for the first time that it is possible to reverse the leukemic blockage of KG1a cells by using both an anti-CD44 mAb and retinoic acid, which may provide a new experimental basis for a differentiative therapy in AML-M0 patients. Expand
Lack of Natural Killer Cell Precursors in Fetal Liver of Ikaros Knockout Mutant Mice
Data suggest that the deficiency of mature NK cells in Ikaros mutant mice is related to lack of functional precursors, and the NK activity values for cells generated from the NK cell precursor followed the same respective relationships found for NK cell precursor frequencies. Expand
Mitogenic activity of gelatin to murine spleen cells.
Tritium thymidine uptake into phytohemagglutinin (PHA)-stimulated spleen cells as well as intactSpleen cells was augmented by gelatin, suggesting that gelatin serves as a mitogen for lymphoid cells and could be used as an agent of cancer biotherapy. Expand
Ligation of the CD44 adhesion molecule reverses blockage of differentiation in human acute myeloid leukemia
It is reported that ligation of CD44 with specific anti-CD44 monoclonal antibodies or with hyaluronan, its natural ligand, can reverse myeloid differentiation blockage in AML1/2 to AML5 subtypes. Expand
HI44a, an anti-CD44 monoclonal antibody, induces differentiation and apoptosis of human acute myeloid leukemia cells.
The results indicate that HI44a effectively induces both differentiation and apoptosis of AML cells and suggest that this activity of the anti-CD44 antibody may be associated with its inhibitory effect on c-myc transcript expression. Expand