Role of 8-epi PGF2alpha, 8-isoprostane, in H2O2-induced derangements of pulmonary artery endothelial cell barrier function.

@article{Hart1998RoleO8,
  title={Role of 8-epi PGF2alpha, 8-isoprostane, in H2O2-induced derangements of pulmonary artery endothelial cell barrier function.},
  author={Craig M Hart and R J Karman and Tiana Blackburn and M. V. N. V. Prasad Gupta and Joe G. N. Garcia and Emile R. Mohler},
  journal={Prostaglandins, leukotrienes, and essential fatty acids},
  year={1998},
  volume={58 1},
  pages={9-16}
}
The non-enzymatic peroxidation product of arachidonic acid, 8-epi-PGF2alpha or 8-isoprostane (8-IP) was measured in H2O2-exposed cultured pulmonary artery endothelial cell (PAEC) monolayers using a commercially-available enzyme immunoassay kit. H2O2 (50 microM for 1-30 min) significantly increased 8-IP production in a time-dependent fashion. Treatment with higher H2O2 concentrations (100 or 250 microM) failed to further increase 8-IP generation. Determinations of thiobarbituric acid reactive… CONTINUE READING
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