Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro.

@article{Sarnow1989RoleO3,
  title={Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro.},
  author={Peter Sarnow},
  journal={Journal of virology},
  year={1989},
  volume={63 1},
  pages={467-70}
}
It has been shown by van der Werf et al. (S. van der Werf, J. Bradley, E. Wimmer, F. W. Studier, and J. Dunn, Proc. Natl. Acad. Sci. USA 83:2330-2334, 1986) that in vitro synthesis of poliovirus RNA by T7 RNA polymerase gives rise to infectious RNA molecules; however, these molecules are only 5% as infectious as RNA isolated from virions. A plasmid, T7D-polio, was constructed that allows the in vitro synthesis of full-length RNA molecules with two additional guanine residues at the 5' end… CONTINUE READING

From This Paper

Topics from this paper.
33 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 33 extracted citations

Similar Papers

Loading similar papers…