Role for nucleolin/Nsr1 in the cellular localization of topoisomerase I.

@article{Edwards2000RoleFN,
  title={Role for nucleolin/Nsr1 in the cellular localization of topoisomerase I.},
  author={Toby Edwards and Ahamed Saleem and Jeffrey A Shaman and Tom Dennis and Christiane Gerigk and Esther Oliveros and Marc R Gartenberg and Eric H. Rubin},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 46},
  pages={36181-8}
}
Nucleolin functions in ribosome biogenesis and contains an acidic N terminus that binds nuclear localization sequences. In previous work we showed that human nucleolin associates with the N-terminal region of human topoisomerase I (Top1). We have now mapped the topoisomerase I interaction domain of nucleolin to the N-terminal 225 amino acids. We also show that the Saccharomyces cerevisiae nucleolin ortholog, Nsr1p, physically interacts with yeast topoisomerase I, yTop1p. Studies of isogenic… CONTINUE READING