Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP)

@inproceedings{Nostrand2016RobustTD,
  title={Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP)},
  author={Eric L. Van Nostrand and Gabriel A. Pratt and Alexander A. Shishkin and Chelsea Gelboin-Burkhart and Mark Yang Fang and Balaji Sundararaman and Steven M. Blue and Thai B. Nguyen and Christine F. Surka and Keri Elkins and Rebecca Stanton and Frank W Rigo and Mitchell Guttman and Gene W. Yeo},
  booktitle={Nature Methods},
  year={2016}
}
As RNA-binding proteins (RBPs) play essential roles in cellular physiology by interacting with target RNA molecules, binding site identification by UV crosslinking and immunoprecipitation (CLIP) of ribonucleoprotein complexes is critical to understanding RBP function. However, current CLIP protocols are technically demanding and yield low-complexity libraries with high experimental failure rates. We have developed an enhanced CLIP (eCLIP) protocol that decreases requisite amplification by ∼1… CONTINUE READING
Highly Influential
This paper has highly influenced 20 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 30 times over the past 90 days. VIEW TWEETS
Blog posts, news articles and tweet counts and IDs sourced by
Altmetric.com