Robust transcriptional signatures for low-input RNA samples based on relative expression orderings

@inproceedings{Liu2017RobustTS,
  title={Robust transcriptional signatures for low-input RNA samples based on relative expression orderings},
  author={Huaping Liu and Yawei Li and Jun He and Qingzhou Guan and Rou Chen and Haidan Yan and Weicheng Zheng and Kai Song and Hao Cai and You Guo and Xianlong Wang and Zheng Guo},
  booktitle={BMC Genomics},
  year={2017}
}
BackgroundIt is often difficult to obtain sufficient quantity of RNA molecules for gene expression profiling under many practical situations. Amplification from low-input samples may induce artificial signals.ResultsWe compared the expression measurements of low-input mRNA samples, from 25 pg to 1000 pg mRNA, which were amplified and profiled by Smart-seq, DP-seq and CEL-seq techniques using the Illumina HiSeq 2000 platform, with those of the paired high-input (50 ng) mRNA samples. Even with… CONTINUE READING
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