Robust analysis of underivatized free amino acids in soil by hydrophilic interaction liquid chromatography coupled with electrospray tandem mass spectrometry.

@article{Gao2016RobustAO,
  title={Robust analysis of underivatized free amino acids in soil by hydrophilic interaction liquid chromatography coupled with electrospray tandem mass spectrometry.},
  author={Jiajia Gao and Rick Helmus and Chiara Cerli and Boris Jansen and Xiang Qing Wang and Karsten Kalbitz},
  journal={Journal of chromatography. A},
  year={2016},
  volume={1449},
  pages={78-88}
}
Amino acids are an important and highly dynamic fraction of organic N in soils and their determination in soil without derivatization is challenging due to the difficulties in separation and detection of trace amounts of these polar analytes. In the present work, we developed an analytical method to quantify 20 free amino acids in aqueous soil extracts without derivatization. The method employed hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) technique… CONTINUE READING