Robust CD4+ and CD8+ T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag.

@article{Melhem2007RobustCA,
  title={Robust CD4+ and CD8+ T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag.},
  author={Nada M Melhem and Xiang Dong Liu and David Boczkowski and Eli Gilboa and Simon M Barratt-Boyes},
  journal={European journal of immunology},
  year={2007},
  volume={37 8},
  pages={2164-73}
}
A potentially powerful strategy for therapeutic HIV vaccination is the use of DC transfected with mRNA encoding autologous viral Ag, as epitopes presented by transfected DC would exactly reflect those expressed by infected cells in the individual. Using human and rhesus macaque monocyte-derived DC, we show that nucleofection is a superior method for mRNA… CONTINUE READING