Robust, reversible gene knockdown using a single lentiviral short hairpin RNA vector.

@article{Brown2010RobustRG,
  title={Robust, reversible gene knockdown using a single lentiviral short hairpin RNA vector.},
  author={Cheryl Brown and Timothy J Sadlon and Tessa Gargett and Elizabeth Melville and Rui Zhang and Yvette Drabsch and Michael Wong Clemente Vui Ling and Craig A. Strathdee and Thomas J Gonda and Simon Charles Barry},
  journal={Human gene therapy},
  year={2010},
  volume={21 8},
  pages={1005-17}
}
Manipulation of gene expression is an invaluable tool to study gene function in vitro and in vivo. The application of small inhibitory RNAs to knock down gene expression provides a relatively simple, elegant, but transient approach to study gene function in many cell types as well as in whole animals. Short hairpin structures (shRNAs) are a logical advance as they can be expressed continuously and are hence suitable for stable gene knockdown. Drug-inducible systems have now been developed… CONTINUE READING

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