Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP).

@article{Nostrand2017RobustCP,
  title={Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP).},
  author={Eric L. Van Nostrand and Thai B. Nguyen and Chelsea Gelboin-Burkhart and Ruth Wang and Steven M. Blue and Gabriel A. Pratt and Ashley L Louie and Gene W. Yeo},
  journal={Methods in molecular biology},
  year={2017},
  volume={1648},
  pages={
          177-200
        }
}
Profiling of RNA binding protein targets in vivo provides critical insights into the mechanistic roles they play in regulating RNA processing. The enhanced crosslinking and immunoprecipitation (eCLIP) methodology provides a framework for robust, reproducible identification of transcriptome-wide protein-RNA interactions, with dramatically improved efficiency over previous methods. Here we provide a step-by-step description of the eCLIP method, along with insights into optimal performance of… CONTINUE READING
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