Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation

@inproceedings{Kappel2012Rlp24AT,
  title={Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation},
  author={Lisa Kappel and Mathias Loibl and Gertrude Zisser and Isabella Klein and Gernot Fruhmann and Christof Gruber and Stefan Unterweger and Gerald N Rechberger and Brigitte Pertschy and Helmut Bergler},
  booktitle={The Journal of cell biology},
  year={2012}
}
Formation of eukaryotic ribosomes is driven by energy-consuming enzymes. The AAA-ATPase Drg1 is essential for the release of several shuttling proteins from cytoplasmic pre-60S particles and the loading of late joining proteins. However, its exact role in ribosome biogenesis has been unknown. Here we show that the shuttling protein Rlp24 recruited Drg1 to pre-60S particles and stimulated its ATPase activity. ATP hydrolysis in the second AAA domain of Drg1 was required to release shuttling… CONTINUE READING