Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells.

@article{Wolffs2005RiskAO,
  title={Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells.},
  author={Petra T. Wolffs and B{\"o}rje Norling and Peter R{\aa}dstr{\"o}m},
  journal={Journal of microbiological methods},
  year={2005},
  volume={60 3},
  pages={315-23}
}
Real-time PCR technology is increasingly used for detection and quantification of pathogens in food samples. A main disadvantage of nucleic acid detection is the inability to distinguish between signals originating from viable cells and DNA released from dead cells. In order to gain knowledge concerning risks of false-positive results due to detection of DNA originating from dead cells, quantitative PCR (qPCR) was used to investigate the degradation kinetics of free DNA in four types of meat… CONTINUE READING
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