Ring of Negative Charge in BK Channels Facilitates Block by Intracellular Mg2+ and Polyamines through Electrostatics

@article{Zhang2006RingON,
  title={Ring of Negative Charge in BK Channels Facilitates Block by Intracellular Mg2+ and Polyamines through Electrostatics},
  author={Yaxia Zhang and Xiaowei Niu and Tinatin I Brelidze and Karl L. Magleby},
  journal={The Journal of General Physiology},
  year={2006},
  volume={128},
  pages={185 - 202}
}
Intracellular Mg2+ and natural polyamines block outward currents in BK channels in a highly voltage-dependent manner. Here we investigate the contribution of the ring of eight negatively charged residues (4 x E321/E324) at the entrance to the inner vestibule of BK channels to this block. Channels with or without (E321N/E324N) the ring of negative charge were expressed in oocytes and unitary currents were recorded from inside-out patches over a range of intracellular Mg2+ and polyamine… CONTINUE READING
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