Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration.

@article{Hafner2018Rif1BA,
  title={Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration.},
  author={Lukas Hafner and Aleksandra Lezaja and Xu Zhang and Laure Lemmens and Maksym Shyian and Benjamin Albert and Cindy Follonier and Jos{\'e} Manuel Nunes and Massimo Lopes and David Shore and Stefano Mattarocci},
  journal={Cell reports},
  year={2018},
  volume={23 4},
  pages={
          983-992
        }
}
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32 Citations

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Despite its evolutionarily conserved function in controlling DNA replication, the chromosomal binding sites of the budding yeast Rif1 protein are not well understood. Here, we analyse genome‐wide…
The role of Rif1 in telomere length regulation is separable from its role in origin firing
TLDR
It is concluded that telomeric origin firing does not cause telomere elongation, and the role of Rif1 in regulating origin firing is separable from its role in regulating telomeres length.
ChECing out Rif1 action in freely cycling cells
TLDR
This study found that Rif1’s regulatory activity at origins is best revealed by an assay that measures replication in unperturbed, freely cycling cultures, as opposed to commonly used protocols in which cells are first blocked in the G1 phase of the cell cycle by mating pheromone, then released into a synchronous S phase.
Temporal control of late replication and coordination of origin firing by self-stabilizing Rif1-PP1 hubs in Drosophila
TLDR
A model in which the spatiotemporal program of late replication in the MBT embryo is controlled by self-stabilizing Rif1-PP1 hubs, whose abrupt dispersal synchronizes firing of associated late origins is proposed.
Dynamic relocalization of replication origins by Fkh1 requires execution of DDK function and Cdc45 loading at origins
TLDR
It is determined that execution of Dbf4-dependent kinase function, including Cdc45 loading, results in dynamic relocalization of a replication origin from the nuclear periphery to the interior in G1 phase, which provides novel molecular insight into the mechanisms that govern dynamics and spatial organization of DNA replication origins and possibly other functional DNA elements.
Telomere length regulation by Rif1 protein from Hansenula polymorpha
TLDR
Functional characterization of the Rif1 homologue from methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1 is reported and it is shown that, similar to other yeast species, H.polymorpha Rif 1 suppresses telomerase-dependent telomere elongation.
Persistent acetylation of newly synthesized histones inhibits DNA replication origin activity
TLDR
The results argue that abnormal regulation of nascent chromatin structure negatively influences DNA replication initiation in cells presenting reduced Dbf4-dependent kinase activity including those experiencing replicative stress.
Rif1 regulates telomere length through conserved HEAT repeats
TLDR
It is proposed that the Rif1 HEAT repeats region represents a protein-protein binding interface that mediates telomere length regulation, which is separate from their proposed DNA-binding function.
Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane
TLDR
Rif1 S-acylation facilitates the accumulation of Rif1 at DSBs, attenuation of DNA end-resection, and DSB repair by non-homologous end-joining by the DHHC family palmitoyl acyltransferase Pfa4.
Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
TLDR
A model on how Rif1 may facilitate the formation of chromatin architecture through its G4 binding and oligomerization properties is presented.
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References

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TLDR
The data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised.
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TLDR
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TLDR
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