Ricin A-chain: kinetics, mechanism, and RNA stem-loop inhibitors.

@article{Chen1998RicinAK,
  title={Ricin A-chain: kinetics, mechanism, and RNA stem-loop inhibitors.},
  author={Xiaoming Chen and Todd M. E. Link and Vern L Schramm},
  journal={Biochemistry},
  year={1998},
  volume={37 33},
  pages={
          11605-13
        }
}
Ricin A-chain (RTA) catalyzes the depurination of a single adenine at position 4324 of 28S rRNA in a N-ribohydrolase reaction. The mechanism and specificity for RTA are examined using RNA stem-loop structures of 10-18 nucleotides which contain the required substrate motif, a GAGA tetraloop. At the optimal pH near 4.0, the preferred substrate is a 14-base stem-loop RNA which is hydrolyzed at 219 min-1 with a kcat/Km of 4.5 x 10(5) M-1 s-1 under conditions of steady-state catalysis. Smaller or… CONTINUE READING
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