Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean.

@article{Buhr2002RibozymeTO,
  title={Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean.},
  author={Tony L. Buhr and Shirley J Sato and Farida Ebrahim and Aiqiu Xing and You Zhou and Michelle Mathiesen and Bruce J. Schweiger and Anthony J. Kinney and Paul Staswick},
  journal={The Plant journal : for cell and molecular biology},
  year={2002},
  volume={30 2},
  pages={
          155-63
        }
}
We investigated whether termination of transcripts with a self-cleaving ribozyme can enhance nuclear retention and serve as a tool to decrease specific plant gene expression. Nuclear retention was first monitored in tobacco using the beta-glucuronidase gene terminated with either the 35S CaMV 3' untranslated sequence (UTR) or a cis-acting ribozyme. Northern blot analysis of nuclear RNA and total RNA, and in situ hybridizations showed that the ribozyme-terminated transcripts were preferentially… CONTINUE READING
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