Ribozyme mediated targeting of cucumber mosaic virus RNA 1 and 2 in transgenic tobacco plants.

@article{Kwon1997RibozymeMT,
  title={Ribozyme mediated targeting of cucumber mosaic virus RNA 1 and 2 in transgenic tobacco plants.},
  author={Chang Seob Kwon and Won Il Chung and Kyung Hee Paek},
  journal={Molecules and cells},
  year={1997},
  volume={7 3},
  pages={
          326-34
        }
}
Hammerhead ribozymes have been extensively used to inhibit the expression of cellular genes or viral genes mainly in the animal study. In this study, we designed a ribozyme targeting the conserved leader sequences of cucumber mosaic virus (CMV) RNA 1 and 2. The ribozyme, with asymmetric lengths of flanking complementary regions, cleaved a model substrate RNA efficiently at 26 degrees C as well as at 37 degrees C or 50 degrees C in vitro. And the ribozyme encoding sequence was introduced into… CONTINUE READING

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