Ribozyme mediated destruction of RNA in vivo.

@article{Cotten1989RibozymeMD,
  title={Ribozyme mediated destruction of RNA in vivo.},
  author={Matt Cotten and Max L. Birnstiel},
  journal={The EMBO journal},
  year={1989},
  volume={8 12},
  pages={3861-6}
}
Previous studies have demonstrated that high ribozyme to substrate ratios are required for ribozyme inhibitory function in nuclear extracts. To obtain high intracellular levels of ribozymes, tRNA genes, known to be highly expressed in most tissues, have been modified for use as ribozyme expression cassettes. Ribozyme coding sequences were placed between the A and the B box, internal promoter sequences of a Xenopus tRNAMet gene. When injected into the nucleus of frog oocytes, the ribozyme tRNA… CONTINUE READING