Ribozyme-based insulator parts buffer synthetic circuits from genetic context


Synthetic genetic programs are built from circuits that integrate sensors and implement temporal control of gene expression. Transcriptional circuits are layered by using promoters to carry the signal between circuits. In other words, the output promoter of one circuit serves as the input promoter to the next. Thus, connecting circuits requires physically… (More)
DOI: 10.1038/nbt.2401


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