Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system.

@article{He2005RibosomeDO,
  title={Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system.},
  author={Mingyue He and Michael J. Taussig},
  journal={Journal of immunological methods},
  year={2005},
  volume={297 1-2},
  pages={73-82}
}
In ribosome display, proteins are linked to their encoding genetic material as protein-ribosome-mRNA complexes. The technology has been applied to the isolation of antibodies and other proteins from large PCR-derived libraries. Here we demonstrate the specificity of eukaryotic ribosome complexes and investigate recovery and display procedures using a single… CONTINUE READING