Ribosome display: cell-free protein display technology.

  title={Ribosome display: cell-free protein display technology.},
  author={Mingyue He and Michael J. Taussig},
  journal={Briefings in functional genomics & proteomics},
  volume={1 2},
Ribosome display is a cell-free system for the in vitro selection of proteins and peptides from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein-ribosome-mRNA (PRM) complexes. This permits the simultaneous isolation of a functional nascent protein, through affinity for a ligand, together with the encoding mRNA, which is then converted and amplified as DNA for further… CONTINUE READING

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