Ribosome Subunit Stapling for Orthogonal Translation in E.  coli

@inproceedings{Fried2015RibosomeSS,
  title={Ribosome Subunit Stapling for Orthogonal Translation in E. 
coli
},
  author={Stephen D. Fried and Wolfgang H. Schmied and Chayasith Uttamapinant and Jason W Chin},
  booktitle={Angewandte Chemie},
  year={2015}
}
The creation of orthogonal large and small ribosomal subunits, which interact with each other but not with endogenous ribosomal subunits, would extend our capacity to create new functions in the ribosome by making the large subunit evolvable. To this end, we rationally designed a ribosomal RNA that covalently links the ribosome subunits via an RNA staple. The stapled ribosome is directed to an orthogonal mRNA, allowing the introduction of mutations into the large subunit that reduce orthogonal… CONTINUE READING
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