Ribosomal proteins cross-linked to the initiator AUG codon of a mRNA in the translation initiation complex by UV-irradiation.

@article{Takahashi2005RibosomalPC,
  title={Ribosomal proteins cross-linked to the initiator AUG codon of a mRNA in the translation initiation complex by UV-irradiation.},
  author={Yoshiaki Takahashi and Sigeru Hirayama and Shoji Odani},
  journal={Journal of biochemistry},
  year={2005},
  volume={138 1},
  pages={
          41-6
        }
}
Eukaryotic ribosomal proteins constituting the binding site for the initiator codon AUG on the ribosome at the translation initiation step were investigated by UV-induced cross-linking between protein and mRNA. The 80S-initiation complex was formed in a rabbit reticulocyte cell-free system in the presence of sparsomycin with radiolabeled Omega-fragment as a template, which was a 73-base 5'-leader sequence of tobacco mosaic virus RNA having AUG at the extreme 3'-terminal end and extended with… CONTINUE READING
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