Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae.

  title={Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae.},
  author={D M Baronas-Lowell and J. R. Warner},
  journal={Molecular and cellular biology},
  volume={10 10},
In the yeast Saccharomyces cerevisiae, L30 is one of many ribosomal proteins that is encoded by two functional genes. We have cloned and sequenced RPL30B, which shows strong homology to RPL30A. Use of mRNA as a template for a polymerase chain reaction demonstrated that RPL30B contains an intron in its 5' untranslated region. This intron has an unusual 5' splice site, C/GUAUGU. The genomic copies of RPL30A and RPL30B were disrupted by homologous recombination. Growth rates, primer extension, and… CONTINUE READING


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