Ribonucleoprotein complex formation during pre-mRNA splicing in vitro.

  title={Ribonucleoprotein complex formation during pre-mRNA splicing in vitro.},
  author={Albrecht Bindereif and Michael R. Green},
  journal={Molecular and cellular biology},
  volume={6 7},
The ribonucleoprotein (RNP) structures of the pre-mRNA and RNA processing products generated during in vitro splicing of an SP6/beta-globin pre-mRNA were characterized by sucrose gradient sedimentation analysis. Early, during the initial lag phase of the splicing reaction, the pre-mRNA sedimented heterogeneously but was detected in both 40S and 60S RNP… CONTINUE READING