Ribonuclease activities in developing experimental granulation tissue with reference to polysomes.

Abstract

The Mg2+-precipitable polysomes in experimental granulation tissue increase up to three weeks and decrease slowly thereafter. Polysomes from young granulation tissue are, on a weight basis, more active in cell-free protein synthesis than polysomes from old granulation tissue. RNase activity is highest in polysomes from 1-week tissue. The polysomes contain both acid and alkaline RNase, but the alkaline RNase is the latent form, manifested by the addition of p-chloromercuribenzoate (pCMB). The high RNase activity of granulation tissue renders the analysis of polysomal patterns and functions of the polysomes difficult. In the post-polysomal supernatant of granulation tissue the RNase activity is highest in 2-4 week old tissue; it is maximal at pH 7-8 and only slightly influenced by pCMB.

Statistics

0200400600800'87'90'93'96'99'02'05'08'11'14'17
Citations per Year

78 Citations

Semantic Scholar estimates that this publication has 78 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Aho1979RibonucleaseAI, title={Ribonuclease activities in developing experimental granulation tissue with reference to polysomes.}, author={Sirpa A. Aho and E I Kulonen}, journal={Acta physiologica Scandinavica}, year={1979}, volume={106 3}, pages={313-8} }