Riboflavin uptake by native Xenopus laevis oocytes.

  title={Riboflavin uptake by native Xenopus laevis oocytes.},
  author={David L. Dyer and Hamid M Said},
  journal={Biochimica et biophysica acta},
  volume={1234 1},
  • D. Dyer, H. Said
  • Published 8 March 1995
  • Biology
  • Biochimica et biophysica acta
Involvement of a receptor-mediated component in cellular translocation of riboflavin.
Data suggest an essential role of microtubule-dependent movement and vesicular sorting component(s) in the bidirectional transport of riboflavin in the presence and absence of pharmacologically active compounds.
Proliferation of peripheral blood mononuclear cells increases riboflavin influx.
Evidence is provided for an energy-dependency relationship between riboflavin uptake and proliferation of peripheral blood mononuclear cells and whether the increase is also mediated by an increased number of transporters per cell.
Riboflavin-Targeted Drug Delivery
This review article summarizes knowledge on RFVT expression in healthy and pathological tissues, discusses riboflavin internalization pathways, and provides an overview of RF-targeted diagnostics and therapeutics.
Riboflavin uptake by the human‐derived liver cells Hep G2: Mechanism and regulation
The mechanism and cellular regulation of RF uptake by a human‐derived liver cellular preparation is described, for the first time, and shows the involvement of a carrier‐mediated system in the uptake process.


Uptake of riboflavin by isolated rat liver cells.
Results showed that overall, entry of riboflavin into hepatocytes occurs predominantly by a facilitated diffusion process followed by rapid trapping by flavokinase-catalyzed phosphorylation to FMN.
Uptake of riboflavin by rat intestinal mucosa in vitro.
The uptake of riboflavin was studied in everted sacs of rat intestine using [14C]riboflavin and [3H]polyethylene glycol to define the mechanism of mucosal membrane transport. Three-minute incubations
In vitro kinetics of the intestinal transport of riboflavin in rats.
The results indicate that it is essential to consider the physiologically relevant concentration of micronutrients in studies concerning the mechanism of their intestinal transport.
Riboflavin uptake by isolated enterocytes of guinea pigs.
Results indicate that riboflavin is absorbed in all parts of the small intestine in an active, carrier-mediated process.
The binding of flavin derivatives to the riboflavin-binding protein of egg white. A kinetic and thermodynamic study.
For most complexes, the binding process is found to be adequately described by a single step equilibrium, allowing the dissociation rate constants for the complexes to be calculated from the equilibrium and association rate constants.
Riboflavin transport by rabbit kidney slices: characterization and relation to cyclic organic acid transport.
  • R. Spector
  • Biology
    The Journal of pharmacology and experimental therapeutics
  • 1982
These studies support the notions that: 1) riboflavin is accumulated in kidney cortex slices by an energy-dependent saturable transport system; 2) rib oflavin accumulation by kidney slices is inhibited by cyclic organic acids and sulfhydryl reagents, but not weak bases; and 3) ribonavin is a competitive inhibitor of aminohippurate as well as an inhibitor of penicillin G accumulation by kidneys slices.
Developmental Maturation of Riboflavin Intestinal Transport in the Rat
The present study demonstrates that the characteristics of the transport process of riboflavin is similar in suckling, weanling, and adult rats and occurs by an energy-, temperature-, and Na+-dependent carrier-mediated process, however, the affinity and the activity of the Transport carriers of rib oflavin decrease with maturation.
Characterization of neutral and cationic amino acid transport in Xenopus oocytes
The characteristics of Na+‐independent uptake of threonine are not consistent with assignment to System L, and appear to be reflective of Systems asc and bo,+, while histidine appears to be transported by a carrier similar in its specificity to System y+, but is taken up by System L when present as a zwitterion.
Effect of probenecid on renal clearance of riboflavin in man.
Renal clearances of riboflavin exceeded (up to three times) the endogenous creatinine clearances, which indicates that rib oflavin excretion involves renal tubular secretion.