Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase.

@article{Santos1995RiboflavinBI,
  title={Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase.},
  author={Maria Angeles Santos and Jos{\'e} Javier Garc{\'i}a-Ram{\'i}rez and Jos{\'e} Luis Revuelta},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 1},
  pages={437-44}
}
Saccharomyces cerevisiae has a monofunctional riboflavin synthase that catalyzes the formation of riboflavin from 6,7-dimethyl-8-ribityllumazine. We have isolated the gene encoding this enzyme from a yeast genomic library by functional complementation of a mutant, rib5-10, lacking riboflavin synthase activity. Deletion of the chromosomal copy of RIB5 led to riboflavin auxotrophy and loss of enzyme activity. Intragenic complementation between point and deletion mutant alleles suggested that the… CONTINUE READING