Rhodopsin in reconstituted phospholipid vesicles. 2. Rhodopsin-rhodopsin interactions detected by resonance energy transfer.

@article{BorochovNeori1983RhodopsinIR,
  title={Rhodopsin in reconstituted phospholipid vesicles. 2. Rhodopsin-rhodopsin interactions detected by resonance energy transfer.},
  author={H Borochov-Neori and P. A. George Fortes and Mauricio Montal},
  journal={Biochemistry},
  year={1983},
  volume={22 1},
  pages={206-13}
}
The interactions between rhodopsin molecules in a micellar detergent solution (octyl glucoside) and in reconstituted phospholipid vesicles were studied in the dark and after bleaching. Resonance energy transfer measurements were used to monitor the proximity between rhodopsin monomers conjugated with a fluorescent donor or a fluorescent acceptor. Reactive sulfhydryl groups of rhodopsin were labeled with pyrenylmaleimide (donor) or monobromobimane (acceptor), whereas amino groups were labeled… CONTINUE READING