Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F

@article{Sheikh1996RhodopsinAB,
  title={Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F},
  author={S\oren Paludan Sheikh and Tatyana A. Zvyaga and Olivier Lichtarge and Thomas P. Sakmar and Henry R. Bourne},
  journal={Nature},
  year={1996},
  volume={383},
  pages={347-350}
}
A LARGE superfamily of receptors containing seven transmembrane (TM) helices transmits hormonal and sensory signals across the plasma membrane to heterotrimeric G proteins at the cytoplasmic face of the membrane. To investigate how G-protein-coupled receptors work at the molecular level, we have engineered metal-ion-binding sites between TM helices to restrain activation-induced conformational change in specific locations. In rhodopsin, the photoreceptor of retinal rod cells, we substituted… CONTINUE READING

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